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Chromogenic Substrates


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Evaluation of Hyphen BioMed Chromogenic Substrates vs Chromogenix Substrates





CHROMOGENIC SUBSTRATES For In Vitro Research Studies.

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The BIOPHEN® line of chromogenic substrates is a new line of  high quality chromogenic substrates, which allow testing enzymes involved in coagulation and fibrinolysis.   The BIOPHEN line of substrates are of the highest quality and stability.   The Hyphen Line of Substrates carry all the traditional substrates for Thrombin, Factor Xa, Factor IIa, Kallikrein, Activated Protein C, Plasmin and Plasminogen-SK, and many more.  To find out the chemical composition of the substrate please click on more information on that substrate.

  • These substrates offer prolonged stability following reconstitution.
  • Aniara can provide substrates in bulk format and for industrial use.
  • Methods have been developed for Factor Xa and Factor IIa assays.
  • We can validate your methods with our reagents – at our lab in France.

Enzymes are proteins that catalyze most of the chemical reactions that take place in the body. They make it possible for chemical reactions to occur at neutral pH and body temperature. The chemical compound upon which the enzyme exerts its catalytic activity is called a substrate. Proteolytic enzymes act on their natural substrates, proteins and peptides by hydrolyzing one or more peptide bond(s). This process is usually highly specific in the sense that only peptide bonds adjacent to certain amino acids are cleaved.

Chromogenic substrates are peptides that react with proteolytic enzymes under the formation of color. They are made synthetically and are designed to possess a selectivity similar to that of the natural substrate for the enzyme. Attached to the peptide part of the chromogenic substrate is a chemical group which when released after the enzyme cleavage gives rise to color. The color change can be followed spectrophotometrically and is proportional to the proteolytic activity.

Chromogenic substrate technology was developed in the early 1970s, and has since then become a tool of substantial importance in basic research. The majority of chromogenic substrate applications are found in various clinical fields. In particular they have been used to generate fundamental knowledge of the mechanisms regulating elastase, mast cell tryptase, and many of the factors regulating blood coagulation and fibrinolysis. Furthermore, products based on chromogenic substrate technology have brought a new generation of diagnostics into the clinical laboratory. These substrates offer a prolonged stability following reconstitution. We can also offer these substrates in 1g and 5g format.

Chromogenic Substrates

Thrombin Substrate

Factor Xa Substrate

Activated Protein C Substrate

Kallicrein Substrate

Plasmin Plasminogen–SK Substrate

Factor IXa Substrate

Urokinase Substrate

tPA and Broad Spectrum Substrate





BIOPHEN CS-01(38) - Thrombin 
  • Peptide Sequence: H-D-Phe-Pip-Arg-pNa, 2HCl
  • Molecular Weight: 552.6 Da
  • Chemical Structure: C27H36N8O5, 2HCl
Chromogenic Substrate for Thrombin
  • Sequence = Tos-Gly-Pro-Arg-pNA.AcOH
  • Molecular weight: 662.6 Da
Synthetic Chromogenic Substrate for Thrombin
BIOPHEN CS-05(88) - tPA & broad spectrum
  • Peptide Sequence: H-D-Ile--Pro-Arg-pNa-2HCl
  • Molecular Weight: 504.6 Da
Synthetic Generic Chromogenic Substrate
BIOPHEN CS-11(32) - Factor Xa
  • Peptide Sequence: Suc-Ile-Gly (γ Pip)Gly-Arg-pNa, HCl
  • Molecular Weight: 760.9 Da
  • Chemical Structure: C34H52N10O10, HCl
Chromogenic Substrate for Factor Xa
BIOPHEN CS-11(65) Anti-Xa
  • Peptide Sequence: D-Arg-Gly-Arg-pNA, 2HCl
  • Molecular Weight: Da
  • Chemical Formula:  C28H39N11O7, 2HCl
Chromogenic Substrate for Factor Xa
BIOPHEN CS-11(22) - Factor Xa
  • Form 1(50%)
  • Peptide Sequence: Bz-Ile-Glu(γ-OCH3)-Gly-Arg-pNA.
  • Molecular Weight: 711.8 Da
  • Chemical Sequence: C33H45N9O9, HCl   
  • Form 2 (50%):
  • Peptide Sequence: Bz-Ile-Glu(γ-OH)-Gly-Arg-pNA 
  • Molecular Weight: 697.7 Da
  • Chemical Sequence: C32H43N9O9, HCl   
Chromogenic Substrate for Factor Xa
BIOPHEN CS-21(66) - Activated Protein C
  • Peptide Sequence: Glu-Pro-Arg-pNa, HCl
  • Molecular Weight: 502.5 Da
  • Chemical Structure: C22H30N8O6, HCl
Chromogenic Substrate for Activated Protein C
BIOPHEN CS-31(02) - Kallicrein
  • Peptide Sequence: D-Pro-Phe-Arg-pNa, 2HCl 
  • Molecular Weight: 538.6 Da
  • Chemical Structure: C26H34N8O5, 2HCl 
Chromogenic Substrate for Kallicrein
BIOPHEN CS-41(03) - Plasmin and Plasminogen-SK
  • Peptide Sequence: PyroGlu-Phe-Lys-pNa, HCl  
  • Molecular Weight: 524.6 Da
  • Chemical Structure: C26H32N6O6, HCl 
Chromogenic Substrate for Plasmin and Plasminogen-SK
BIOPHEN FIXa CS 51(09) - Factor IXa
  • Peptide Sequence: CH3SO2-(D-)CHG-Gly-Arg-pNa, AcOH
  • Molecular Weight:  628.7 Da    
Chromogenic Substrate for Factor IXa
BIOPHEN CS-61(44) Urokinase
  • Peptide Sequence: pyro-Glu-Gly-Arg-pNa.HCl
  • Molecular weight: 462.5 Da
  • Chemical Structure: C19H26N8O6,  HCl.
Synthetic Generic Chromogenic Substrate


  • Peptide Sequence: Suc-Ile-Gly (γ Pip)Gly-Arg-AMC     
  • Molecular Weight: 798 Da
Synthetic Fluorognic substrate for testing factor Xa